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### Access Dataset can be accessed and downloaded from the directory via: [http://arcticdata.io/data/10.18739/A2599Z39H](http://arcticdata.io/data/10.18739/A2599Z39H). ### Overview This dataset contains raw sedimentary ancient DNA (sedaDNA) sequence data and associated metadata generated from a 3-meter sediment core collected from Andy Lake, Alaska. The core was obtained during fieldwork supported by the National Science Foundation (NSF) Arctic Doctoral Dissertation Research Improvement Grant #2230019, titled Assessing the impacts of Pleistocene megaherbivores on vegetation stability and resilience in the Arctic, awarded to Principal Investigator (PI) Jacquelyn L. Gill and Co-PI Alessandro Mereghetti. The primary objective of this project is to investigate how Pleistocene megafaunal diversity influenced long-term plant community dynamics and ecosystem resilience in Arctic environments. These data contribute to understanding ecological baselines and biotic responses to past climate and extinction events, with implications for modern conservation and rewilding strategies. 

Open top chambers (OTCs) were adopted as the recommended warming mechanism by the International Tundra Experiment (ITEX) network in the early 1990’s. Since then, OTCs have been deployed across the globe. Hundreds of papers have reported the impacts of OTCs on the abiotic environment and the biota. Here we review the impacts of the OTC on the physical environment, with comments on the appropriateness of using OTCs to characterize the response of biota to warming. The purpose of this review is to guide readers to previously published work and to provide recommendations for continued use of OTCs to understand the implications of warming on low stature ecosystems. In short, the OTC is a useful tool to experimentally manipulate temperature, however the characteristics and magnitude of warming varies greatly in different environments, therefore it is important to document chamber performance to maximize the interpretation of biotic response. When coupled with long-term monitoring, warming experiments are a valuable means to understand the impacts of climate change on natural ecosystems.